The authors of this site use real time PCR routinely in their laboratories. We decided to post our experiences from establishing real time PCR in our laboratories because we spent much time testing a wide variety of equipment, reaction mixes and consumerables and wanted to share the results with a wider audience, hoping that other researchers could save valuable time setting up the method in their laboratories.
The authors of these pages are:
Ying C Lee, PhD
Senior scientist at the Institute of Cellular Biology, University of Copenhagen.
Main field of interest: Intracellular signaling in insulin producing beta cells.
André Campos, MD
Post-graduate student at the Bartholin Institute during med school.
Has worked on projects involving quantification of gene activity by real time PCR.
Knud Josefsen, MD, PhD
Senior scientist at the Bartholin Institute, University Hospital; Copenhagen.
Main field of interest: Genetic regulation of the insulin secretion in pancreatic beta cells
